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. 2015 Jan 20;13(1):681–696. doi: 10.3390/md13010681

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the chitosanase produced by B. cereus TKU033. Lanes: M, molecular markers (170, 130, 95, 72, 55, 43, 34, 26, 17, and 10 kDa); (1) crude enzyme; (2) adsorbed chitosanase fractions after DEAE-Sepharose CL-6B chromatography; (3) adsorbed chitosanase fractions after Macro-prep DEAE chromatography.