Table 1. Coverage necessary to reach a 99.9% probability of recovering three copies of the correct sequence for all alleles (based on the proportion of correct reads). Since not all alleles in a PCR amplify equally well, we calculated the coverage needed when two alleles amplify at the same rate (equal amplification), and when one allele yields twice as many products as the other (unequal amplification). Enzymes that did not amplify are marked n.a. and those which amplified but for which there was insufficient data to calculate coverage are labeled i.d. Abbreviations are those used in Figures 1 and 2 and the text.
| Enzyme | Abbreviation | Test 1 | Test 2 equal amplification | Test 2 unequal amplification |
|---|---|---|---|---|
| Phusion® High Fidelity DNA Polymerase (Finnzymes) | Phusion | 7 | 42 | 87 |
| KAPA HiFi™ (Kapa Biosystems) | KapHF | 7 | i.d. | i.d. |
| Pwo® DNA Polymerase (Roche) | Pwo | 7 | n.a. | n.a. |
| AmpliTaq Gold® (Applied Biosystems) | Gold | 9 | 48 | 88 |
| i-MaxTM II DNA Polymerase (iNtRON Biotechnology) | iMax | 11 | 57 | 99 |
| Taq DNA Polymerase (Roche) | Roche Taq | 11 | 120 | 185 |
| Velocity DNA Polymerase (Bioline) | Velocity | 12 | n.a. | n.a. |
| HotStarTaq® DNA Polymerase (Qiagen) | HotStar | 14 | 97 | 152 |
| FastStart® High Fidelity PCR System (Roche) | FastStart | 14 | 45 | 86 |
| Biotaq® (Bioline) | Biotaq | 16 | 271 | 395 |
| OneTaq™ DNA Polymerase (New England Biolabs) | OneTaq | i.d. | n.a. | n.a. |
| Vent® DNA Polymerase (New England Biolabs) | Vent | n.a. | n.a. | n.a. |
| Deep Vent® DNA Polymerase (New England Biolabs) | DeepVent | n.a. | n.a. | n.a. |