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. 2015 Jan 27;5:8039. doi: 10.1038/srep08039

Figure 1. Viral polymerase activity in vitro.

Figure 1

Human A549 and avian DF-1 cells were transfected with plasmids encoding the PB1, PA, NP, and wild-type or mutant PB2, with a plasmid for the expression of a virus-like RNA encoding the firefly luciferase gene (driven by a human or an avian polymerase I promoter for use in human or avian cells, respectively), and with a control plasmid encoding Renilla luciferase. Cells were incubated for 24 h at 37°C (A549) or 39°C (DF-1), and then firefly and Renilla luciferase activities were measured by means of a dual-luciferase assay. Polymerase activity was calculated by normalization of the firefly luciferase activity to the Renilla luciferase activity. The data are shown as relative polymerase activities with SD (n = 3). The polymerase activity of the wild-type Anhui/1 PB2 was set to 100%. **, P < 0.01, according to a one-way ANOVA followed by a Dunnett's test.