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. 2015 Jan 27;5:8039. doi: 10.1038/srep08039

Figure 2. Growth kinetics of mutant viruses in vitro.

Figure 2

Human A549 (A) and avian DF-1 (B) cells were infected with the indicated viruses at a multiplicity of infection of 0.001 and incubated at 37°C or 39°C, respectively. The supernatants of the infected cells were collected at the indicated time points. Virus titers were determined by use of a plaque assay in MDCK cells. The virus titers are means ± SD (n = 3). * and **, P < 0.05 and P < 0.01, respectively, according to a one-way ANOVA followed by a Dunnett's test.