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. 2015 Jan 27;5:8053. doi: 10.1038/srep08053

Figure 3. Characteristics of liver tumors harvested from 2PLEASE mice.

Figure 3

(A) A typical focus of altered hepatocytes detected in the liver of a 2PLEASE mouse (H&E staining, upper left panel) already presenting as a mass forming hepatocellular adenoma. Higher magnification (upper middle panel) reveals enlarged hepatocytes with small lipid vacuoles. Massive glycogen storage is evident in the PAS staining (upper right panel). Another example of a glycogen-storing focus in the liver (PAS staining, lower left panel). Fluorescence imaging shows GFP expression in cells in the nodule (lower middle panel), confirming transgene expression following Cre-mediated DNA excision. Beneath the region containing green fluorescent cells are normal hepatocytes that are GFP negative. Hepatocytes in this focus are also PAS-reactive (lower right panel). Scale bars, 250 μm for left panels and 50 μm for middle and right panels. (B) Comparison of mRNA levels of selected genes. Quantitative RT-PCR was performed to compare the expression levels of the indicated genes between normal liver tissue (white bar) and liver tumors (black bar). Relative expression levels are shown. Single asterisks indicate p < 0.05 and double asterisks indicate p < 0.01.