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. 2015 Feb 10;22(5):350–361. doi: 10.1089/ars.2014.5891

FIG. 2.

FIG. 2.

Effect of GSSG on CBS. (a) S-glutathionylation was carried out by treating CBS [0.5 mg/ml with 1 mM DA/1.5 mM GSH (lane 2), 1 mM GSSG (lane 3), 2 mM GSSG (lane 4), or 5 mM GSSG (lane 5) followed by nonreducing SDS-PAGE and Western blot analysis] with anti-SSG antibody (top) or anti-CBS antibody (bottom). (b) Effect of GSSG on CBS activity. CBS (0.5 mg/ml) was incubated with the indicated concentrations of GSSG for 1 h at room temperature, and then the samples were dialyzed to remove GSSG. CBS activity was measured using the methylcysteine assay. The graph represents the treated compared with untreated controls and is the average±SD (n=3). GSSG, glutathione disulfide.