Table 4.
Anaplasmataceae species detected in ticks from Ecuador
| Bacterium (no.) | Host/sampling | No. and stage of tick species | Gene | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| msp2 | 16S rRNA | 16S rRNA (EHR) | 16S rRNA (GEP) | Pan-bacterial 16S rRNA | ||||||||
| Maximum % identity (bp) | GenBank acc. no. | Maximum % identity (bp) | GenBank acc. no. | Maximum % identity (bp) | GenBank acc. no. | Maximum % identity (bp) | GenBank acc. no. | Maximum % identity (bp) | GenBank acc. no. | |||
| A. phagocytophilum or closely related genotypes (10) | Tapir | 1F A. multipunctum | 99.3 (290/292) | CP000235 | ND | ND | ND | NP | ||||
| Farm 1, Cow 1 | 3F R. microplus | 96.6-98.3 (280-285/290) | FJ600595 | ND | ND | ND | NP | |||||
| 2F R. microplus | 97.9 (284/290) | CP000235 | ND | ND | ND | NP | ||||||
| 1F R. microplus | 96.6 (282/292) | AY164493 | 100 (497/497) | JF893938 | ND | ND | NP | |||||
| 1F R. microplus | 96.5 (278/288) | AY164493 | ND | ND | ND | NP | ||||||
| 1F R. microplus | 96.9 (281/290) | FJ600595 | 100 (497/497) | JF893938 | ND | ND | NP | |||||
| Vegetation | 1M A. multipunctum | 99.3 (289/291) | AY626255 | ND | ND | ND | NP | |||||
| A. marginale (2) | Farm 1, Cow 2 | 1F R. microplus | ND | ND | 100 (305/305) | CP001079 | 100 (297/297) | CP001079 | 100 (1129/1129) | CP000030 | ||
| JN558818 | JN558818 | |||||||||||
| DQ648489 | EU436153 | |||||||||||
| CP001759 | ||||||||||||
| Farm 1, Cow 3 | 1F R. microplus | ND | ND | 100 (305/305) | CP001079 | 100 (297/297) | CP001079 | 100 (1129/1129) | CP000030 | |||
| JN558818 | JN558818 | |||||||||||
| DQ648489 | EU436153 | |||||||||||
| CP001759 | ||||||||||||
| Anaplasma spp. (3) | Farm 1, Cow 2 | 1M R. microplus | ND | ND | 100 (305/305) | CP001079 | ND | 99.4 (1222/1229) | JQ480818* | |||
| JN558818 | ||||||||||||
| DQ648489 | ||||||||||||
| CP001759 | ||||||||||||
| Farm 1, Cow 3 | 1M R. microplus | ND | ND | 100 (305/305) | CP001079 | 100 (297/297) | CP001079 | 99.4 (1222/1229) | JQ480818* | |||
| JN558818 | JN558818 | |||||||||||
| DQ648489 | EU436153 | |||||||||||
| CP001759 | ||||||||||||
| Farm 2, Cow 1 | 1F R. microplus | ND | ND | ND | 100 (297/297) | CP001079 | 99.4 (1222/1229) | JQ480818* | ||||
| JN558818 | ||||||||||||
| EU436153 | ||||||||||||
*Coxiella endosymbiont of Rhipicephalus turanicus isolate DGGE.
A. phagocytophilum: Anaplasma phagocytophilum; A. multipunctum: Amblyomma multipunctum; A. marginale: Anaplasma marginale; R.: Rhipicephalus; M: male; F: Female; ND: Not detected; NP: Not performed; CP001079-CP000030: Anaplasma marginale sequences from GenBank; JN558818: Anaplasma ovis sequence from GenBank; DQ648489-EU436153: Anaplasma phagocytophilum sequences from GenBank; CP001759: Anaplasma centrale sequence from GenBank.