Skip to main content
. 2015 Jan 7;12:1. doi: 10.1186/s12983-014-0093-6

Figure 2.

Figure 2

Co-staining of MIP and phalloidin in 1 month post fertilization (mpf) Platynereis. (A-K) Immunostaining of 1mpf Platynereis with an antibody raised against Platynereis MIP7 (red), counterstained with phalloidin. (A) Full body ventral view. White boxes indicate areas examined in cross-section in (B-D). Yellow arrowheads in (A-D) indicate cell bodies of MIP-expressing neurons. (B-D) Apical views of 10 μM cross-sections of the foregut (B), foregut-midgut boundary (C) and hindgut (D). (E) Ventral view of mid- and hindgut with ventral nerve cord region removed to expose digestive system. White box indicates area examined in cross-section in (F). Yellow arrowheads in (E-H, J) indicate cell bodies of MIP-expressing neurons in the midgut. Orange and purple arrowheads in (E, G, H) mark cell bodies of MIP-expressing neurons in the hindgut. (F) Apical view of 10 μM cross-section in the midgut. Dashed white line marks the boundary between the gut lumen and epithelial cell layer. (G, H) Lateral layers of (E) indicating the MIP-expressing cell bodies of the midgut and hindgut that sit in the gut epithelium, just underlying the gut musculature. (I-K) Close-up ventral view of the mid- and hindgut. (I) Phalloidin staining shows the circular and longitudinal smooth muscle fibres of the gut. (J) MIP immunostaining. (K) Overlay of MIP immunostaining and phalloidin staining. The axons of MIP-expressing neurons run parallel to the muscle fibres of the gut. Scale bars in (A, E, G-K): 50 μM, in (B-D, F): 20 μM. Abbreviations: fg, foregut; mg, midgut; hg, hindgut; VNC, ventral nerve cord; DIC, differential interference contrast; cm, circular muscle fibre; lm, longitudinal muscle fibre.