Table 2.
Specific flour protein types that increased or decreased in relative amounts when non-transgenic and transgenic plants were grown with post-anthesis fertilizer
| %Change with Fertilizer 1 | |||
|---|---|---|---|
| Protein Type 2 | Control | 35b | 45a |
| HMW-GS By9 | 32.0 | 35.2 | 25.8 |
| LMW-GS Bu-11 (m-type) | −13.4 | −17.9 | −21.2 |
| LMW-GS Bu-18 (m-type) | −5.3 | −9.6 | −21.4 |
| LMW-GS [GenBank: AAB48469] (i-type) | 13.5 | 24.7 | 17.7 |
| Omega-1,2 chain-terminating gliadin3 | 89.2 | 93.3 | 26.3 |
| Alpha gliadin Bu-5 | 49.8 | 41.7 | 30.5 |
| Alpha gliadin Bu-11 | 32.6 | 32.0 | 23.6 |
| Alpha gliadin Bu-12 | 38.1 | 38.5 | 27.1 |
| Alpha gliadin Bu-14 | 32.1 | 32.6 | 28.3 |
| Alpha gliadin Bu-27 | 24.0 | −18.8 | 8.6 |
| Alpha gliadin Bu-BQ807130 | 29.5 | 11.6 | 17.9 |
| Omega-5 gliadin | 70.7 | 3.3 | −3.9 |
| Omega-1,2 gliadin D34 | 31.6 | 26.9 | 4.3 |
| Omega-1,2 gliadin Bu-D54 | 79.5 | 66.0 | 19.1 |
| Secalin-like omega gliadin | 31.5 | 24.5 | 14.1 |
| Triticin | 15.4 | 15.5 | 21.3 |
| Farinin Bu-1 | −29.9 | −39.7 | −43.1 |
| Farinin Bu-2 | −30.8 | −31.0 | −28.1 |
| Purinin Bu-1 | −11.9 | −25.4 | −26.9 |
| Purinin Bu-2 | −12.8 | −22.4 | −32.1 |
| Purinin Bu-3 | −7.3 | −11.0 | −24.4 |
| Serpin Bu-1 | 49.6 | 55.9 | 31.6 |
| Serpin Bu-2 | 103.7 | 118.2 | 69.3 |
| Serpin Bu-3 | 54.3 | 63.8 | 60.3 |
| Serpin Bu-4 | 56.3 | 60.2 | 53.2 |
| Serpin Bu-5 | 50.7 | 51.1 | 47.2 |
| Serpin Bu-7 | 26.3 | 11.9 | 16.2 |
| WMAI Bu-1 | −24.8 | −26.0 | −31.1 |
| WDAI Bu-1 | −17.9 | −25.2 | −25.8 |
| WDAI Bu-4 | −27.4 | −41.6 | −38.8 |
| WTAI CM1 | −17.9 | −26.1 | −31.1 |
| WTAI CM2 | −26.0 | −34.8 | −30.9 |
| WTAI CM3 | −26.0 | −34.0 | −38.9 |
| WTAI CM16 | −27.7 | −35.7 | −37.0 |
| WTAI CM17 | −30.6 | −39.7 | −39.8 |
| WCI | −30.0 | −38.1 | −39.6 |
| CMX1/CMX3 | −23.0 | −28.7 | −31.0 |
| WASI | −29.1 | −25.8 | −28.0 |
1Protein types that either increased or decreased at least 20% in response to post-anthesis fertilizer are indicated in bold. Changes greater than 20% that were not deemed to be significant by ANOVA are shown in bold italics.
2Only protein types that changed in response to fertilizer in at least one line are shown.
3Protein has an amino acid sequence similar to an omega-1,2 gliadin but contains one cysteine that enables it to be linked into the glutenin polymer.
4Two distinct protein types were distinguished for omega-1,2 gliadins. Percent changes of total omega-1,2 gliadins with fertilizer were 47.7, 39.3 and 7.6 for the non-transgenic, 35b and 45a, respectively.