Figure 1.
tRNAs and precursor tRNA from T. acidophilum. The sequences of initiator tRNAMet (A), elongator tRNAMet (B) and tRNALeuUAG (C) are depicted by cloverleaf structures. The regions to which the 3’-biotinylated DNA probes hybridize are illustrated. The abbreviations are as follows: pseudouridine, ψ; N2-methylguanosine, m2G; 2'-O-methylcytidine, Cm; 1-methyladenosine, m1A; 4-thiouridine, s4U; unknown modification, N; unidentified G modification, G*; N6-thereonylcarbamoyladenosine, t6A; archaeosine, G+; N2,N2-dimethylguanosine, m22G; 5-carbamoylmethyluridine, ncm5U; 1-methylguanosine, m1G; 7-methylguanosine, m7G. The precursor of elongator tRNAMet contains an intron at the canonical site (D). Triangles show the cleavage sites of tRNA splicing endonuclease.