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Activities of tRNA methyltransferases in the crude cell extracts. The activities of tRNA methyltransferases in the S-30 (A) and S-100 (B) fractions were analyzed by 2D-TLC. 14C-methylated nucleotides were monitored by autoradiography. The solvent systems were as follows: first dimension, isobutyric acid, ammonia, water, 66/1/33 v/v/v; second dimension, isopropyl alcohol, HCl, water, 70/15/15 v/v/v.
