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. 2014 Dec 24;16(1):178–192. doi: 10.3390/ijms16010178

Figure 6.

Figure 6

Down-regulated ARPP-19 resulted in decreased phosphorylated mitotic substrates. (A) The protein levels of ARPP-19, cyclin A, cyclin D1, cyclin B1, Cdk2 and Cdk4 in HepG2 or SMMC-7721 cells were analyzed by western blot, with β-actin serving as an internal reference; (B) HepG2 or SMMC-7721 cells were synchronized at the M phase by thymidine and released into nocodazole for 16 h. The protein levels of ARPP-19, phospho-cdc2 (Tyr15) and the phosphorylation of the different cyclin B-Cdc2 substrates (phospho-(Ser) CDKs substrate) were analyzed by western blot, with β-actin serving as an internal reference; (C) Quantification of data in (B). All data are shown as the mean ± SEM of three independent experiments, and significant values are indicated with asterisks (* p < 0.05; ** p < 0.01, versus scramble).