Skip to main content
. 2014 Dec 30;16(1):645–659. doi: 10.3390/ijms16010645

Figure 2.

Figure 2

Activation of caspases and subsequent cleavage of PARP during the morin-induced apoptosis in U937 cells. U937 cells were incubated at indicated concentrations of morin for 48 h. (A) Western blot analysis for the effects of morin on the caspase activation and PARP cleavage. The membranes were probed with the anti-caspase-3, anti-caspase-8, anti-caspase-9 and anti-PARP antibodies. The expression of the indicated proteins were measured by densitometry and expressed as average relative ratio compared to actin, from two or three different experiments and (B) In vitro assay for caspase-3, -8 and -9 activity, which uses DEVD-pNA, IETD-pNA and LEHD-pNA as substrates, respectively. The released fluorescent products were measured. Data are expressed as mean ± SD of three independent experiments. (* p < 0.05 vs. control).