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. 2014 Dec 31;16(1):840–856. doi: 10.3390/ijms16010840

Figure 3.

Figure 3

Comparison of the high-throughput sequencing and quantitative real-time PCR results. (a) The differentially expressed miRNAs, including rno-miR-183-5p, rno-miR-9a-5p, rno-miR-199a-5p, rno-miR-351-5p, rno-miR-200b-3p, rno-miR-191a-3p, rno-miR-181c-3p, rno-miR-330-5p rno-miR-126a-5p and rno-miR-351-3p were used to perform expression analyses by qPCR. Comparisons of the high-throughput sequencing and qRT-PCR results indicated that the two plarforms exhibited good correlation; and (b) The 5p/3p ratio validation results for miR-126a, miR-330 and miR-351 by qPCR also indicated good correlation between the two platforms.