Table 1.
Protein | MW, kD | 1/MW, kD−1 | Kd(1), nM | Kd(2), nM | N |
---|---|---|---|---|---|
IgG | 150 | 0.00667 | 4 | 30 | 52 |
BSA | 66 | 0.01515 | 6 | 76 | 90 |
Protein G | 26 | 0.03846 | 10 | 175 | 500 |
STI | 20 | 0.05 | 15 | 100 | 550 |
Kd(1) and Kd(2) are the equilibrium dissociation constants for sections of the Scatchard graph significantly below the saturation value, and close to the saturation value, respectively; N is the maximum number of binding sites; BSA: bovine serum albumin; STI: Kunitz-type soybean trypsin inhibitor; protein G: recombinant streptococcal protein G; IgG: human immunoglobulin G.