Table 3.
Primary and secondary antibodies use for Western blot analysis.
| Antibody | Corporation and Product Number | Dilution Rate |
|---|---|---|
| COX-2 | Cell signaling technology #4842 (Beverly, MA, USA) | 1:5000 |
| iNOS | Cell signaling technology #2977 | 1:1000 |
| HO-1 | Santa Cruz biotechnology #SC-10789 (Santa Cruz, CA, USA) | 1:500 |
| NF-κB p65 | Cell signaling technology #3034 | 1:1000 |
| P-IκBα | Cell signaling technology #2859 | 1:1000 |
| IκBα | Cell signaling technology #4814 | 1:1000 |
| β-actin | Santa Cruz biotechnology #SC-47778 | 1:5000 |
| TBP | Santa Cruz biotechnology #SC-33736 | 1:1000 |
| P-ERK | Cell signaling technology #4377 | 1:1000 |
| ERK | Cell signaling technology #9102 | 1:1000 |
| P-p38 | Cell signaling technology #9211 | 1:1000 |
| p38 | Cell signaling technology #9212 | 1:1000 |
| P-JNK | Cell signaling technology #9251 | 1:1000 |
| JNK | Cell signaling technology #9252 | 1:1000 |
| Secondary anti-mouse | Thermo scientific #31437 (Hudson, NH, USA) | 1:5000 |
| Secondary anti-rabbit | Thermo scientific #31463 | 1:5000 |