Table 4.

Primers used for RT-PCR analysis.

Target Gene		Primer Sequence		Annealing Temperature
TNF-α		F: 5'-AGCACAGAAAGCATGATCCG-3'		55 °C
		R: 5'-GTTTGCTACGACGTGGGCTA-3'
IL-6		F: 5'-CATGTTCTCTGGGAAATCGTGG-3'		58 °C
		R: 5'-AACGCACTAGGTTTGCCGAGTA-3'
IL-1β		F: 5'-TGCAGAGTTCCCCAACTGGTACATC-3'		64 °C
		R: 5'-GTGCTGCCTAATGTCCCCTTGAATC-3'
COX-2		F: 5'-CACTCAGTTTGTTGAGTCATTC-3'		45 °C
		R: 5'-GATTAGTACTGTAGGGTTAATG-3'
iNOS		F: 5'-AGCCCAACAATACAAATGACCCTA-3'		56 °C
		R: 5'-TTCCTGTTGTTTCTATTTCCTTTGT-3'
HO-1		F: 5'-TGAAGGAGGCCACCAAGGAGG-3'		62 °C
		R: 5'-AGAGGTCACCCAGGTAGCGGG-3'
β-actin		F: 5'-ATGAAGATCCTGACCGAGCGT-3'		58 °C
		R: 5'-AACGCAGCTCAGTAACAGTCCG-3'

F, forward; R, reverse.