Figure 4.

The endocytic internalization process. Endocytosis sites are first initiated by the recruitment of clathrin via the AP-2 adaptor complex (early coat) to cluster cargo proteins to the endocytic invagination sites at the plasma membrane (PM). Initiation is terminated by the recruitment of ANTH- (AP180 N-terminal homology) and ENTH- (epsin N-terminal homology) domain containing proteins Sla2 and epsins Ent1 and Ent2 to form the late coat. Invagination starts by the recruitment of the WASP/Myosin module to initiate actin branched polymerization by the actin module (Arp2/3 complex and actin). Myosin is squeezing the PM while the actin module expands the invagination. Once the invagination in long enough, the fission module starts pinching off vesicles that are released into the cytoplasm.