Expression of miRs-143, -145, -7, -34a, and -21 in human colorectal tumor tissues were evaluated by performing TaqMan® Real-time PCR assays. The relative expression levels were calculated by the ΔΔCt method, with RNU6B used as a control. The relative expression level in normal tissue was indicated as “1”. The expression levels in tumors were designated as down-regulated when the fold change from the expression in the non-tumorous tissue was 0.67 or as up-regulated when the fold change from the expression in the non-tumorous tissue was 1.50. Statistical differences in miRNA levels were evaluated by using Pearson’s χ2 test or Fisher’s extract test for differences between two groups. A p-value of 0.05 was considered to be significant; statistical analysis was done in this manner for all subsequent Tables.