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. 2015 Jan 9;16(1):1562–1575. doi: 10.3390/ijms16011562

Figure 3.

Figure 3

Specificity of the LAMP assay for the authentication of T. formosanum (A). The LAMP reactions were performed using specifically designed LAMP primers of TF. Lane M, 1 kb of DNA marker; lane N, without plant genomic DNA; lanes 1, Taraxacum formosanum; lane 2, Taraxacum officinale; lane 3, Ixeridium laevigatum; lane 4, Youngia japonica; lane 5, Ixeris chinensis; lane 6, Emilia sonchifolia var. javanica; Sensitivity analysis of the LAMP assay for the authentication of T. formosanum (B). The LAMP reaction was performed using various quantities of genomic TF DNA as template. The upper panels represent LAMP products detected by DNA electrophoresis; the lower panels represented LAMP products detected by SYBR Green I staining and UV excitation. Lane 1, 100 ng; lane 2, 20 ng; lane 3, 1 ng; lane 4, 100 pg; lane 5, 10 pg; lane 6, 1 pg; lane 7, 100 fg; lane 8, 10 fg; lane 9, 1 fg.