Table 1.
The sequences of primers used for SYBR Green real-time PCR of genes involved in glycogen metabolism.
| Gene | Primers Used for R. microplus Genes | Amplicon Size (bp) | Gene Used as Reference * | GenBank Access Number of R. microplus Gene |
|---|---|---|---|---|
| Hexokinase | CATGGACAAAGAGCTTCAACTGCTC GGAAAGCTCCCTTGACCAGGGTA |
150 | XM_002412706.1 Ixodes scapularis | KF951259 |
| Pyruvate kinase | GGGCAAGAGGGCAAGACAACTG CACGTTGAGCACCTTGGTGATG |
141 | XM_002407420.1 Ixodes scapularis | KF951260 |
| Phophoenolpyruvate carboxykinase | CAAGCAATGAGTGCCTGCCAC ACAGTCTTCCGTTTTCATCTTG |
147 | XM_002413329.1 Ixodes scapularis | KF951261 |
| Glucose-6-phosphatase | GGCAGCCATTTGGTACATCATCC CGACAGGCTGACAATGCACAGG |
133 | XM_002407091.1 Ixodes scapularis | KF951262 |
| Glycogen synthase-6 | GCTGGTATCGGGCTGATCCTG GATGCCTCTGTCTCCAGCCTCC |
165 | XM_002435718.1 Ixodes scapularis | KF951264 |
| Phosphoglucomutase | CGGATCTGGGCAAGCTGGG CCGTCGTGACCCTTGATGAGG |
151 | XM_003695907.1 Apis florea | KF951265 |
| Triose phosphate isomerizes | CCTCGCTGCACAAAATTGCTAC TCCGAATGACCCAGTATGACCC |
128 | EF014474 | |
| Protein kinase B | GGCCAAAGCCATTCACCTTCA CCTCCTCACTCGCCAACTTCTC |
151 | JX648548 | |
| Glycogen synthase kinase 3 | CCCACACCCGCTATTTATTG TGTGCAGGAGAGCCAGTTTA |
113 | EF142066 | |
| Glycogen-debranching enzyme | ATGCTCAGGATCACGCAGAAGC GTACGTCGGTTGGGAAGGACAAGG |
173 | XM_002401176.1 Ixodes scapularis |
* Access numbers of published DNA sequences used to conduct Blast searches for matching gene transcripts in the R. microplus database (PRJNA232001 TSA database).