Figure 4.

Relative content of phosphorylated Ca²⁺/CaM Kinase II, Protein Kinase C and Extracellular Signal-Regulated Kinase 1/2 in hippocampal slices incubated with Melatonin. Hippocampal slices were incubated for 3 h with either the vehicle (VEH) or 100 nM melatonin (MEL). CaMKII and Thr286-phospho-CaMKII (P-CaMKII), PKC and phospho-PKC (P-PKC) as well as ERK1/2 and phospho-ERK1/2 (P-ERK1/2) were determined by Western blot. Upper panels show immunodetection of GAPDH as load control. Representative fluorograms of total and phosphorylated enzymes revealed by ECL are shown below. Histograms correspond to densitometric analysis of bands depicted immediately above. Results are expressed as the mean ± SEM of four densitometric scannings obtained from two independent experiments. Asterisks indicate significant differences (p < 0.05).