Table 3.
Primers used to analyze transcript levels of CYP genes in T. castenuem.
| Primers | Sequence (5'–3') | Tm (°C) | Product Length (bp) |
|---|---|---|---|
| TcCYP12H1-F | AACCGCAAAAACTGATACGG | 60.0 | 299 |
| TcCYP12H1-R | ACCGGTCGTGTCTATTCCTG | 60.0 | |
| TcCYP4G7-F | CGCTGCCAACAGAGACATTA | 60.0 | 207 |
| TcCYP4G7-F | AATGACCCTGAAACCGTCAG | 60.0 | |
| TcCYP4BR3-F | CATCGGTTGTACCCTCCTGT | 59.9 | 168 |
| TcCYP4BR3-R | GAATCGGTCAGGGTCAAAGA | 59.8 | |
| TcCYP4Q4-F | TGGTTCCAATCACCCAATTT | 60.0 | 203 |
| TcCYP4Q4-R | TTTTTGCTCTTTGCGACCTT | 59.5 | |
| TcCYP345A1-F | TTTTTCGATTTTCGGTGGAG | 60.0 | 120 |
| TcCYP345A1-R | TTCGCGAAGGAAGTTGCTAT | 60.0 | |
| TcCYP6BK11-F | GTCAATTTGCGGAAACAGGT | 60.1 | 167 |
| TcCYP6BK11-R | CTACGTCCGTAAACCCGAAA | 60.3 | |
| TcCYP9D4-F | GTGGCACAACTAGCTCCACA | 59.9 | 172 |
| TcCYP9D4-R | GTTTTCCTTTACGGGCTTCC | 60.0 | |
| TcCYP9Z5-F | AGTCATGCAAAACTGCAACG | 59.9 | 250 |
| TcCYP9Z5-R | GTCCGGATTGGGGAAGTATT | 60.0 | |
| TcRPS3-F | CCGTCGTATTCGTGAATTGACTT | 59.3 | 143 |
| TcRPS3-R | TCTAAGAGACTCTGCTTGTGCAATG | 60.8 |
F: Forward; R: Reverse.