Table 1.
Gene | Primer sequence (5’-3’) | Primer length (bp) | Product (bp) | Ata (°C) |
---|---|---|---|---|
α1-Na+-K+ATPase | (F) 5’-AGATTTGAGCCGAGGCCTAACACC-3’ | 24 | 418 | 58 |
(R) 5’-TCCGCCCTTCACCTCCACCAGAT-3’ | 23 | |||
β1-Na+-K+ATPase | (F) 5’-GCCCCGCCAGGATTGACAC-3’ | 19 | 444 | 58 |
(R) 5’-CTCATCGCGCTTGCCAGTG-3’ | 19 | |||
β-actin | (F) 5’-GTACAACCTTCTTGCAGCTCCT-3’ | 22 | 871 | 55 |
(R) 5’-ACAGGATTCCATACCCAGGAAG-3’ | 22 |
AT = annealing temperature.