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Flow cytometry analysis of levels of CAR and integrins αvβ3 and αvβ5 in experimental cell lines. Cells were labeled with mouse anti-CAR monoclonal antibody, mouse anti-human integrin αvβ3 (LM609) antibody or mouse anti-human integrin αvβ5 (P1F6) antibody. The cells were then incubated with FITC-conjugated rabbit anti-mouse IgG secondary antibody and analyzed by flow cytometry.
