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. 2014 Dec 15;7(12):4951–4958.

Figure 2.

Figure 2

The expression of EMT phenotype marker induced by TNF-α treatment. TNF-α upregulates mesenchymal markers expression and downregulates epithelial markers expression. A: mRNA levels for E-cadherin, Vimentin, Slug and ZEB1 in untreated (control) and TNF-α treated in ACHN cell line after 4 days of treatment; B: mRNA levels for E-cadherin, Vimentin, Slug and ZEB1 in untreated (control) and TNF-α treated in 786-0 cell line after 4 days of treatment; C: Western blotting analysis for E-cadherin, Vimentin, Slug and ZEB1 in untreated (control) and TNF-α treated in ACHN cell line after 4 days of treatment; D: Western blotting analysis for E-cadherin, Vimentin, Slug and ZEB1 in untreated (control) and TNF-α treated in 786-0 cell after 4 days of treatment. *P < 0.05 compared to Control.