Table 1.
Genes | Forward primer/reverse primer |
---|---|
E-cadherin | 5’-AGAGGGTCACCGCGTCTATG-3’ |
5’-CTCACAGGTGCTTTGCAGTT-3’ | |
Vimentin | 5’-TCCAGCAGCTTCCTGTAGGT-3’ |
5’-CCCTCACCTGTGAAGTGGAT-3’ | |
Slug | 5’-TGTTGCAGTGAGGGCAAGAA-3’ |
5’-GACCCTGGTTGCTTCAAGGA-3’ | |
ZEB1 | 5’-GCCAATAAGCAAACGATTCTG-3’ |
5’-TTTGGCTGGATCACTTTCAAG-3’ | |
Oct 4 | 5’-GTCCGAGTGTGGTTCTGTA-3’ |
5’-CTCAGTTTGAATGCATGGGA-3’ | |
Nanog | 5’-CAAAGGCAAACAACCCACTT-3’ |
5’-TCTGCTGGAGGCTGAGGTAT-3’ | |
Bmi-1 | 5’-CTGGTTGCCCATTGACAGC-3’ |
5’-CAGAAAATGAATGCGAGCCA-3’ | |
18S | 5’-GGAATTGACGGAAGGGCACCACC-3’ |
5’-GTGCAGCCCCGGACATCTAAGG-3’ |