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SIRT1 had the properties of modulating cellular senescence in GC cells. GES-1 (A), AGS (B) and MKN-45 (C) were treated with indicated methods. Then, cellular senescence was assayed by SA-β-gal assay. The relative fluorescence intensity was normalized as the percentage of β-gal activity from normal cells. All experiments were performed in triplicate. The data represented the mean ± SEM. The statistical results were indicated by asterisks and **represents P < 0.01.
