Figure 4.

The effects of SIRT1 on viability and senescence were involved in STAT3 and NF-κB. AGS cells were knocked down by using siRNA for STAT3 (STAT3si) and siRNA for NF-κB p65 (NF-κBsi) respectively. The effectiveness of genes knockdown was assessed by western plot (C). The si-control group represented the cells with Non-Target shRNA. Then AGS cells or AGS cells with indicated treatments were incubated with RSV or NA for 24 h. Cellular viability (A) and senescence (B) were measured by CCK-8 assay and SA-β-gal assay respectively in differ groups. All experiments were performed in triplicate. The data represented the mean ± SEM.