Figure 2.

(A) 3′UTR of CBX7 is a target of miR-9 predicted by TargetScan and PicTar. (B) The luciferase reporter assay results, with each bar representing values from 3 independent experiments. The transfection efficiency was normalized by co-transfected Renilla luciferase and the light units were calculated by relative luciferase activity of firefly to Renilla. * P<0.01. (C) Representative image of the protein level of CBX7. GAPDH was used as a reference control. (D) Quantitative analysis of the relative protein levels of CBX7 normalized to those of GAPDH is shown. Data are mean ± SD of 3 independent experiments. * P<0.01.