Fig. 4. Mechanisms of siRNA delivery by DOTMA-OA LNPs.
(A) A cryo-TEM image of nanostrucure of siRNA-DOTMA based LNPs containing OA. White arrows show the onion-like structure of LNPs. (B) Stability of DOTMA based LNPs containing EggPC, OA, LA and LNA. (C) Measurement of pH-dependent zeta potential of DOTMA based LNPs. (D) Co-localization study of DOTMA-OA-Cy3-siRNA (red) with lysotracker (green). Red arrows indicate the co-localization. Scale bar=20 μm.