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. 2014 Dec 31;11:218. doi: 10.1186/s12974-014-0218-z

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© Kinoshita et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Pretreatment of ouabain (OUA) (1.8 μg/ kg) in lipopolysaccharide (LPS) (200 μg/ kg ) inflammation model induced no changes on Na,K-ATPase (NKA) activity. The total NKA activity was tested by adding 10 μg of the particulate fraction from the rat hippocampus obtained 2 hours after OUA (1.8 μg/kg, ip) or saline pretreatment + LPS (200 μg/kg, ip). α_2/3-NKA activity was measured by subtracting the activity obtained with 3 μM OUA (it inhibits just these 2 isoforms) from total NKA activity. To determine the α₁ subunit-associated NKA activity, the total NKA activity was subtracted from the activity obtained with 3 mM OUA (Mg-ATPase). Results are expressed as nmol/mg.min protein (mean + SEM) from five individual experiments. One-way ANOVA followed by Newman-Keuls post-test.