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. 2014 Dec 31;11:218. doi: 10.1186/s12974-014-0218-z

Figure 3.

Figure 3

Effects of pretreatment of ouabain (OUA) (1.8 μg/ kg) on lipopolysaccharide (LPS)-induced (200 μg/kg ) p65 (RELA) subunit NF- κB nuclear translocation and IκB (IKB) degradation. Pretreatment of OUA blocks the LPS-induced NF-κB activation. (A) Representative Western blotting autoradiographs and densitometric analysis (arbitrary units, AU) of p65 nuclear/β-ACTIN ratio, *P < 0.05 versus control and OUA + LPS. (B) Representative Western blotting autoradiographs and densitometric analysis (arbitrary units, AU) of IKB cytosolic/β-ACTIN ratio, *P < 0.05 versus control, OUA, OUA + LPS. Data are presented as mean ± SEM from five individual experiments. One-way ANOVA followed by Newman-Keuls post-test.