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. 2015 Jan 9;22:4. doi: 10.1186/s12929-014-0108-9

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© Subramanian et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Expression of EpCAM post silencing using PEI Nanocomplexes. A. qPCR analysis, graph showing the fold change in the EpCAM expression levels, calculated by normalizing with untreated controls and using GAPDH as internal control gene. qPCR was performed post treatment with PEI alone or PEI-Apt-siRNA complexes or silencing using Lipo-siEp. B. Immunoblots were performed for EpCAM and beta-actin to check the silencing efficiency at protein level using EpCAM C-10 primary antibody from Santa Cruz at 1:400 dilution, secondary anti- mouse IgG HRP from Santacruz at 1:1000 dilution. C. Densitometry of the immunoblot normalized with tubulin-internal control and untreated control. D. Cell proliferation was assessed by performing MTT assay. The graph represents the percentage cell proliferation after treatment with the aptamer alone or PEI alone or PEI-Apt-siRNA complexes. Percentage cell proliferation was calculated considering untreated cells as controls in MCF-7 &WERI-Rb1 cell lines.