Table 2.
X/Y ratio of Type A and Type B clones
| Type A clone | Arithmetic mean X/Y ratio | Standard deviation [%] | Type B clone | Arithmetic mean X/Y ratio | Standard deviation [%] |
|---|---|---|---|---|---|
| His-LOV | 1.7 | 7.1 | I12C | 0.6 | 0.5 |
| Wild type enzyme (His-LOV-BSLA) | 2.2 | 4.6 | F17P | 0.6 | 3.6 |
| A1W | 2.2 | 7.9 | G93Y | 0.5 | 6.7 |
| H10D | 1.4 | 2.0 | V99K | 0.5 | 0.4 |
| K23stop | 1.5 | 2.9 | L102W | 0.4 | 4.5 |
| S56P | 1.9 | 3.8 | K170E | 0.4 | 3.3 |
| D91R | 1.4 | 2.5 | |||
| S167P | 2.4 | 7.5 | |||
| G175F | 1.4 | 1.8 | |||
| Type A | 1.4 – 2.4 | Type B | 0.4 – 0.6 | ||
| Type A* | >1.2 | Type B* | <1.2 |
Ratio between integral X (representing protein production phase) and integral Y (representing second growth phase) determined for the 15 investigated E. coli BL21(DE3) clones according to Figure 4. For each clone, the X/Y ratio was calculated for all individual cultivations presented in Figure 3. The arithmetic mean as well as the standard deviation (in %) are presented.
*Critical X/Y ratio for simplified classification between Type A and Type B clones.