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. 2015 Jan 27;10(1):e0116773. doi: 10.1371/journal.pone.0116773

Fig 2. Effect of TNF on dexamethasone-induced gene expression in BEAS-2B cells.

Fig 2

Human bronchial epithelial, BEAS-2B, cells were pre-treated for 1 h with 10 ng/ml of tumor necrosis factor-α (TNF), before addition of 1 μM dexamethasone (Dex). Cells were harvested 6 h after Dex addition. Total RNA was extracted, reverse transcribed to cDNA and RT-PCR performed for: cyclin-dependent kinase inhibitor 1C (CDKN1C; p57KIP2), dual specificity phosphatase 1 (DUSP1; MKP1), regulator of G-protein signaling 2 (RGS2), TSC22 domain family member 3 (TSC22D3; GILZ) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data (n = 7), normalized to GAPDH, are expressed as fold and plotted as means ± S.E. Significance was tested using repeated measures, one-way analysis of variance (ANOVA) with Bonferroni’s correction for multiple comparisons. ***, P<0.001.