Figure 11. The effect of the p21^cip1 genotype on beta-actin expression.

The graph shows the amount of beta-actin detected per cell expressed as a percentage change relative to the empty vector negative control. Beta-actin expression was determined by Acumen Cytometry with beta-actin immunostaining: with the total amount of beta-actin detected divided by the total number of cells in the sample. The cells were not double stained for p21^cip1 protein, so we were unable to differentiate the p21^cip1 positive population (transfected cells) from the p21^cip1 negative population (non-transfected cells). X-axis: EV NC: Empty vector negative control; com p21: cells transfected with common p21^cip1; var p21: cells transfected with variant p21^cip1. The y-axis represents the percentage change in beta-actin expression per cell relative to the EV NC. The top of the bars represent the mean. The error bars represent the SEM.