Table 1.
% of secondary structural fractions from the far-UV CD spectra of three BoNT/A protein forms- (a) BoNT/A holotoxin (b) BoNT/A complex and (c) NAPs complex at different pH conditions estimated using the method of Yang et al.
| pH | (a) BoNT/A holotoxin | (b) BoNT/A complex | (c) NAPs | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| α-helix (%) | β-sheet (%) | β-turn (%) | RC (%) | α-helix (%) | β-sheet (%) | β-turn (%) | RC (%) | α-helix (%) | β-sheet (%) | β-turn (%) | RC (%) | |
| 7.2 | 18.6 ± 0.8 | 42.0 ± 6.6 | 18.3 ± 3.0 | 21.2 ± 2.7 | 14.0 ± 1.3 | 32.4 ± 0.6 | 17.5 ± 3.4 | 36.4 ± 5.6 | 7.2 ± 1.9 | 61.5 ± 3.5 | 6.9 ± 0.1 | 24.6 ± 2.5 |
| 2.5 | 18.4 ± 2.6 | 37.9 ± 2.7 | 20.1 ± 1.2 | 23.5 ± 1.2 | 17.1 ± 3.5 | 35.3 ± 2.8 | 18.0 ± 0.3 | 29.6 ± 0.4 | 9.1 ± 0.8 | 51.7 ± 1.9 | 9.6 ± 1.0 | 30.1 ± 1.7 |
| 2.5–7.2 | 29.8 ± 4.1 | 3.8 ± 0.4 | 32.7 ± 0.2 | 34 ± 0.3 | 11.8 ± 0.9 | 46.9 ± 3.5 | 13.9 ± 2.8 | 27.4 ± 0.1 | 7.2 ± 1.3 | 58.0 ± 12.3 | 7.9 ± 6.2 | 26.6 ± 5.0 |