Table 2.
Estimation of the values of the melting temperature (Tm) and the pseudo thermodynamic parameters-ΔH, ΔS and ΔG from the thermal denaturation profiles of (a) BoNT/A holotoxin (b) BoNT/A complex and (c) NAPs complex using the Van’t Hoff equation. The Tm (°C) was measured as the mid-point of the S-shaped portion of the thermal denaturation curve.
| pH | (a) BoNT/A holotoxin | (b) BoNT/A complex | (c) NAPs | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ΔH (kJ/mol) | ΔS (J/mol.K) | ΔG (kJ/mol) | Tm (°C) | ΔH (kJ/mol) | ΔS (J/mol.K) | ΔG (kJ/mol) | Tm (°C) | ΔH (kJ/mol) | ΔS (J/mol.K) | ΔG (kJ/mol) | Tm (°C) | |
| 7.2 | 587.0 ± 36.6 | 1782.9 ± 112.2 | 57.9 ± 3.2 | 54.6 ±0.2 | 348.3 ± 40.4 | 1005.7 ± 117.2 | 48.6 ± 5.4 | 74.2 ±0.2 | 378.8 ± 9.1 | 993.7 ± 89.4 | 82.7 ± 35.8 | 78.8 ± 0.3 |
| 2.5 | - | - | - | - | 532.8 ± 5.2 | 1552.0 ± 15.4 | 70.1 ± 0.6 | 71.1 ± 0.3 | 506.5 ± 53.7 | 1468.8 ± 156.4 | 68.8 ± 7.1 | 73.4 ± 0.2 |
| 2.5–7.2 | 823.9 | 2508.9 | 76.3 | 55.0 ± 0.2 | 271.2 ± 54.6 | 782.3 ± 157.6 | 38.1 ± 7.6 | 74.2 ± 0.2 | 313.3 ± 15.2 | 922.2 ± 74.7 | 38.5 ± 7.1 | 78.8 ± 0.3 |