Fig. 2.
Co-localization of μ (MOR), δ (DOR), and κ (KOR) opioid receptors with Sox2+ and Nkx2.2+ glial precursors at 2-3 DIV (A-I) and 5 DIV (J-P). Opioid receptors are widely expressed by subpopulations of Sox2+/Nkx2.2+ glial precursors (A-P). Note, MOR, Nkx2.2 and Sox2 immunoreactivity can be localized to the same cell (arrow in M-P) and overlapping Sox2 and Nkx2.2 immunoreactivity was evident in some DOR or KOR expressing cells (data not shown); scale bar = 20 μm (A,D,G); scale bar = 25 μm (B-C,E-F,H-I); scale bar = 10 μm (J-P).