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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Ultrastruct Pathol. 2014 Sep 30;39(1):69–77. doi: 10.3109/01913123.2014.950778

Figure 3.

Figure 3

FISH analysis performed on a representative FFPE tissue section using a custom-designed dual color TFE3 break-apart probe set demonstrated separation of the green (centromeric to the 5′TFE3 gene locus at Xp11.2) and orange (telomeric to the 3′TFE3 locus) signals with loss of the 5′TFE3 green signal indicative of an unbalanced rearrangement of TFE3 (A). Similarly, separation of the 5′TFE3 (orange) and 3′TFE3 (green) signals with loss of the 5′TFE3 orange signals was observed with the three-color probe set (B). The three-color probe set also demonstrated the presence of additional copies of the X chromosome (aqua signals) and extra 3′TFE3 signals (arrows) (B). A fusion of the TFE3 and ASPSCR1 (17q25.3) loci was confirmed (arrows) using a probe to the 3′ region of TFE3 (green) and a probe spanning ASPSCR1 (orange) with DXZ1 as an X chromosome control probe (aqua) (C).