Dorsal skin of mice was exposed topically to either NM (3.2 mg) in 200 μL acetone or 200 μL of acetone alone. 24, 72 and 120 h post-NM exposure, liver was collected, processed, sectioned and subjected to H&E staining as detailed under ‘Materials and Methods’ section. NM-induced changes in the size and weight of the liver were recorded (A and B) and liver histopathology was analyzed (representative pictures, C). VC, vehicle control (acetone); NM, nitrogen mustard-exposed mice; PT, portal triad; CV, central vein; black arrows, dilated sinusoids; red scale bar, 1 cm; black scale bar (histology images), 100 μm. Data represent mean ± SEM of three-eight animals in each treatment group. *, p < 0.05.