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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2014 Nov 6;7(2):152–168. doi: 10.1002/wnan.1314

Figure 1.

Figure 1

A surface plasmon resonance (SPR) can be excited in thin gold film using the Kretschmann configuration (a, S: Source, D: Detector) and detected as a sharp decrease in the intensity of the reflected beam occurring at the SPR angle or wavelength (b). Film SPR is often used to study biomolecular interactions (c). Target analyte binding by the film-immobilized receptors is detected by a shift in the film SPR. This SPR shift can be monitored over time as target analyte is introduced into the flow cell and then washed away. Kinetic parameters describing the molecular interaction can be calculated from the resulting SPR sensorgram (d, Reproduced with permission from Ref 6. Copyright 2000 Elsevier)