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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Genesis. 2014 Sep 17;53(1):194–201. doi: 10.1002/dvg.22820

Figure 5. Expression patterns of markers for germline and germline-associated cells in B. schlosseri.

Figure 5

(a) vasa is expressed by clusters of germ cells associated with the secondary bud (dashed line) and just posterior to the secondary bud at stage B2. (b) At stage B1, piwi-positive germ cells are present posterior to the secondary buds (dashed lines). (c-d) tgfβ-f expression is detected in clusters of primitive follicle cells posterior to the secondary bud (dashed lines) at stage A2 in non-fertile juveniles (c) and in follicle cells surrounding the maturing oocytes (o) and testes (t) in the primary buds of fertile animals (d). (e-f) Two-color FISH can be used to simultaneously visualize vasa expression (red) and tgfβ-f expression (green) in non-fertile (e) and fertile (f) animals. Scale bars indicate 50 μm.