Fig. 1.

Histone H1-T-Sapphire, SepK-GFP, An-Ndc80 localization in mitosis. Images are three-dimensional projections of Z-series stacks collected at 30 sec intervals at 25°C. An-Ndc80 overlaps with the Nud1 homolog SepK (an SPB marker) in interphase (A-D). In prophase and metaphase as the SPBs separate, An-Ndc80 is between the SPBs (E-L). Individual KTs are visible at metaphase/anaphase (M-P). (Since chromosomes do not form a distinct metaphase plate in A. nidulans it is difficult to distinguish metaphase from very early anaphase.) In Q-T An-Ndc80, and thus the KTs, have moved to the poles. Anaphase A, chromosome to pole movement, is thus complete but chromatids have not visibly separated. Chromosomal separation occurs as the poles move further apart (U-X).