Figure 8.
DA neurons labeled by the C′-GAL4 are candidates for promoting a preference for laying eggs on the sucrose substrate. A, Females with their C′-GAL4-expressing neurons activated preferred the sucrose substrate for egg laying. N = 29, 17, 36, 25, 17, 27 for columns, left to right. B, Females with their C′-GAL4-expressing neurons inhibited preferred the plain substrate for egg laying. N = 29, 32, 20, 28, 31, 25 for columns, left to right. The following abbreviations are used for A and B: (1) C′/+: C′-GAL4/+, (2) C′ > tsh-GAL80, dTRPA1: tsh-GAL80, UAS-dTRPA1/+, C′-GAL4/+, and (3) C′ > tsh-GAL80, shiTS: tsh-GAL80/+; C′-GAL4/UAS-shibireTS. C, C′-GAL4 labeled neurons (in green) sparsely in the adult brain (counterstained with HRP). Blue rectangle, PAL cluster; pink rectangle, PPL2 cluster. D—D″, C′-GAL4 neurons in the PAL cluster coexpressed TH. D, C′-GAL4 neurons labeled by mCD8-GFP. D′, The same neurons stained with anti-TH antibody. D″, Merged image. Note that neurons in these images are different from those shown in C. E—E″, C′-GAL4 neurons in the PPL2 cluster coexpressed TH. E, C′-GAL4 neurons labeled by mCD8-GFP. E′, The same neurons stained with anti-TH antibody. E″, Merged image. Note that neurons in these images are different from those shown in C. F, Comparison of the numbers of DA neurons in the SOG, PPL2, and PAL clusters that are labeled by TH-GAL4, C′-GAL4 and Ddc(HL5)-GAL4, respectively. For each GAL4, six brains were examined. GAL4-expressing neurons were scored by mCD8-GFP expression. DA neurons were scored by TH antibody staining. S, sucrose; P, plain; PI, Preference Index.