Selective ablation and inhibition of MB neurons did not affect females' appraisal of sucrose substrate for egg laying. A, A representative picture showing that MB was intact in the brains of untreated animals (34/34). B, A representative picture showing that MB was largely ablated in the brains of HU-treated animals (34/35). C, HU-treated and untreated WT flies showed similar egg-laying preferences for the plain substrate. N = 30 for each group. D, HU-treated and untreated TH-activated flies showed similar egg-laying preferences for the sucrose substrate. N = 41, 45, 68, 68 for columns, left to right. E, Inhibiting the output of MB neurons did not affect females' preference for laying eggs on the plain substrate. The following abbreviations are used: (1) 247/+: 247-LexA/+, (2) shiTS/+: LexAop-shibireTS/+, and (3) 247 > shiTS: LexAop-shibireTS/+; 247-LexA/+. N = 27, 25, 45, 30, 28, 51 for columns, left to right. F, Inhibiting the output of MB neurons did not affect TH-activated females' preference for laying eggs on the sucrose substrate. The following abbreviations are used: (1) TH*: UAS-dTRPA1/+; TH-GAL4/+ and (2) TH*, 247 > shiTS: LexAop-shibireTS/+; UAS-dTRPA1/+; TH-GAL4/247-LexA. N = 40, 25, 67, 20 for columns, left to right. S, sucrose; P, plain; PI, Preference Index.