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. 2015 Jan 28;35(4):1659–1674. doi: 10.1523/JNEUROSCI.2925-14.2015

Figure 2.

Figure 2.

Expression of TERT protein in the hippocampus at different Braak stages. A, Appropriately sized bands represent hTERT protein (127 kDa) and βIII-tubulin (55 kDa) as a loading control. Graph represents densitometric analysis of relative band densities for hTERT protein. Data are mean ± SEM for 3 cases per group, analyzed using one-way ANOVA. B, Graph represents levels of hTERT protein determined using a TERT ELISA. Data are mean ± SEM from 6 cases per group, analyzed using one-way ANOVA. C, Hippocampal sections from age-matched controls, and from three AD groups, Braak I-II, III, and VI, were labeled with anti-TERT antibody (green) and a fluorescent Nissl stain (NeuroTrace; red). Graph represents quantification of TERT immunofluorescence in neurons from five different areas of the hippocampus: CA1, CA2, CA3, CA4, and DG. Data are mean ± SEM from 3 cases per group, analyzed using one-way ANOVA. D, Two representative fluorescence microscopy images show TERT and NeuroTrace-labeled neurons in the CA1 of a control donor and a Braak Stage VI donor. Scale bars, 50 μm.