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. 2015 Jan 28;35(4):1659–1674. doi: 10.1523/JNEUROSCI.2925-14.2015

Figure 4.

Figure 4.

Levels of mitochondrial proteins are unchanged in AD. A, Western blots for mitochondrial proteins were performed with lysates from controls and from AD cases (Braak Stage VI). There were no significant differences in levels of VDAC/porin or MTCO1 protein (n = 5 or 6, p > 0.05). Data are mean ± SEM and were analyzed using a two-sample t test. B, Histogram showing quantification of MTCO1-immunolabeled hippocampal sections. There are no significant differences in fluorescence intensity between control and AD cases (n = 3, p > 0.05). Scale bars, 50 μm. C, Mouse liver mitochondria were isolated, then subfractionated into mitoplast, proteinase K-shaved mitoplast, and inner membrane fractions. Western blots show appropriately sized bands representing mTERT (127 kDa) and the mitochondrial marker proteins: AIF (67 kDa; matrix/inner mitochondrial membrane), TOM20 (20 kDa; outer mitochondrial membrane/intermembrane space), MnSOD (25 kDa; matrix), and NDUFA9 (36 kDa; inner mitochondrial membrane). TERT protein expression is found in all fractions. H, Liver homogenate; M, pure mitochondria; MP, mitoplast; IM, inner mitochondrial membrane.