Figure 5.
Ih blockade of HCN channels in CBs modulates frequency of spontaneous depolarizations. A, Current-clamp recording from ON CB in response to injected current in the absence and presence of Ih antagonist ZD7288. Arrows, hyperpolarizing sag and rebound depolarization indicative of Ih. B, Current-clamp recordings from an OFF CB in response to injected current in the absence and presence of ZD7288. C, Concurrent current-clamp recordings of spontaneous depolarizations in an AII and OFF RGC in whole-mount retina in the absence and presence of ZD7288. Note that the AII and the OFF GC remain anti-correlated but that the hyperpolarization of the AII in the interwave interval is abolished by ZD7288. Control recording same as Figure 2. D, Summary data of effects on interwave interval distributions. Dotted lines are control distributions from Figure 2.